| Primer | Species | LP | RP | Tml | Tmr | Length | Score | Target | Senstive |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Leptospira biflexa | ACAGTGATTCCCGTTCCTGG | GCCACGTACCATCAATGTCC | 59.68 | 58.99 | 162 | 171 |
100.00%
|
100.00%
|
| Primer 2 | Leptospira biflexa | AGTGGAGCGGTAGTTGTGG | TTGCCCTTTCACTCCGTGC | 59.33 | 60.90 | 172 | 168 |
100.00%
|
100.00%
|
| Primer 3 | Leptospira biflexa | AGGGACAGAAGCTGCGATTC | CCGGTTTTCCATGGCATTGG | 60.11 | 60.11 | 281 | 162 |
100.00%
|
100.00%
|
| Primer 4 | Leptospira biflexa | TGGCAATTCCCACAAGGTG | TTCATCGAGCCCACCATCC | 58.26 | 59.47 | 228 | 162 |
100.00%
|
100.00%
|
| Primer 5 | Leptospira biflexa | TGCCGTGCTTGGTTTTGG | CCGCACTTGTCACCTTCAG | 59.19 | 58.76 | 218 | 159 |
100.00%
|
100.00%
|
| Primer 6 | Leptospira biflexa | AAGGAGGGACAGAAGCTGC | CCGGTTTTCCATGGCATTGG | 59.32 | 60.11 | 285 | 157 |
100.00%
|
100.00%
|
| Primer 7 | Leptospira biflexa | TGCCTCTGGGAAAACGGTG | CCGCAACGGTTTTCGCAT | 60.23 | 59.75 | 221 | 149 |
100.00%
|
100.00%
|
| Primer 8 | Leptospira biflexa | ACAGTGATTCCCGTTCCTGG | TGCCACGTACCATCAATGTCC | 59.68 | 60.68 | 163 | 149 |
100.00%
|
100.00%
|
| Primer 9 | Leptospira biflexa | GCCTCTGGGAAAACGGTGT | CCGCAACGGTTTTCGCAT | 60.23 | 59.75 | 220 | 148 |
100.00%
|
100.00%
|
| Primer 10 | Leptospira biflexa | TTCCATCCCCTCCCGATGT | GGACAAACTGTATGCTTCGGC | 60.00 | 59.87 | 179 | 143 |
100.00%
|
100.00%
|